Team:Warsaw/Calendar-Main/25 June 2008

From 2008.igem.org

(Difference between revisions)
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  Elongation time 1 - 2 minutes.</li>
  Elongation time 1 - 2 minutes.</li>
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<li>We have successfully amplified Alpha-link and link-A. Now its time for PCL (Polymerase Chain Ligation) to create fusions  Alpha-A and Omega-A (Fig.1.).</li></ol>
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<li>We have successfully amplified Alpha-link and link-A. Now its time for PCL (Polymerase Chain Ligation) to create fusions  Alpha-A and Omega-A (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/25_June_2008#fig1">Fig. 1</a>).</li></ol>
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<img src="https://static.igem.org/mediawiki/2008/8/89/PCR_gradient_Alinker_WAW.jpg" width=400/>
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<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/8/89/PCR_gradient_Alinker_WAW.jpg" width=400/></a>
<var> <b>Fig. 1.</b> PCR product - link-A.</var>
<var> <b>Fig. 1.</b> PCR product - link-A.</var>

Revision as of 11:39, 12 October 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

Repetition of PCRs and gel-out isolations from 16 June.

PCR of Alpha-link and link-A

Michał L. Marcin and Ewa

  1. We've ran gradient PCR to obtain Alpha-link and link-A.
    Annealing temp 48°C - 55°C.
    Elongation time 1 - 2 minutes.
  2. We have successfully amplified Alpha-link and link-A. Now its time for PCL (Polymerase Chain Ligation) to create fusions Alpha-A and Omega-A (Fig. 1).
Fig. 1. PCR product - link-A.

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