Team:Warsaw/Calendar-Main/21 May 2008

From 2008.igem.org

(Difference between revisions)
Line 2: Line 2:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
-
<html><h3>Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7</h3>
+
<html><h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5+AID-T7</a></h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
<p><ol>
<p><ol>

Revision as of 15:18, 12 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

  1. Inoculation of separate transformant colonies for transcriptional fusion (liquid LB + tetracycline).
  2. Inoculation of separate transformant colonies for translational fusion (liquid LB + tetracycline).

Results of rifampicin test

Michał L., Ewa, Marcin

StrainInducer / repressorNumber of colonies on rifampicin
Top10none12
Top101mM IPTG17
Top10 pMPM-AIDnone13
Top10 pMPM-AID0.1% Glucose18
Top10 pMPM-AID0.1% Arabinose54
Top10 pTRC-AIDnone14
Top10 pTRC-AID1% Glucose17
Top10 pTRC-AID0.1% Arabinose53

Conclusion: AID seems to work!

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/21_May_2008"