"
Team:Warsaw/Calendar-Main/25 June 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
|||
| Line 6: | Line 6: | ||
<h3>Preparation of constructs with OmpA protein fusions</h3> | <h3>Preparation of constructs with OmpA protein fusions</h3> | ||
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
| - | <p>Repetition of PCRs and gel-out | + | <p>Repetition of PCRs and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out purifications</a> from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_June_2008">16 June.</a> |
</p> | </p> | ||
| Line 18: | Line 18: | ||
Elongation time 1 - 2 minutes.</li> | Elongation time 1 - 2 minutes.</li> | ||
| - | <li>We have successfully amplified Alpha-link and link-A. Now | + | <li>We have successfully amplified Alpha-link and link-A. Now it's time for PCL (Polymerase Chain Ligation) to create fusions Alpha-A and Omega-A (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/25_June_2008#fig1">Fig. 1</a>).</li></ol> |
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/8/89/PCR_gradient_Alinker_WAW.jpg" width=400/></a> | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/8/89/PCR_gradient_Alinker_WAW.jpg" width=400/></a> | ||
Revision as of 15:46, 12 October 2008
 |
|
||||||||
 |
|
||||||||
Preparation of constructs with OmpA protein fusionsMichał K.Repetition of PCRs and gel-out purifications from 16 June. PCR of Alpha-link and link-AMichał L. Marcin and Ewa
Fig. 1. PCR product - link-A.
|
|
