Team:Warsaw/Calendar-Main/12 May 2008

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Latest revision as of 16:11, 12 October 2008


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Preparation of pMPMT5+AID construct

Michał K.

E. coli TOP10 electroporation with ligation (pMPMT5+AID).
Plating on LB + tetracycline.

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+<html><h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5+AID</a> construct</h3>
-<p>Michał K.:</p>
+<h4>Michał K.</h4>
-<p>1. Restriction digest of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a> with HindIII and NcoI (1x Tango buffer).</p>
-<p>2. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation of DNA</a> encoding AID from proper band.</p>
+<p>
+<ol>
+<li> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#electrotransform">electroporation</a> with ligation (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5+AID</a>).</li>
-<p>3. Restriction digest of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> with HindIII and NcoI, use of alkaline phosphatase (CIAP).</p>
+<li>Plating on LB + tetracycline.</li></ol></p>
-<p>4. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> vector from proper band.</p>
+</html>
-<p>5. DNA ligation (pMPM-T5+AID vector)</p>
-<html><div align="center"><img src="http://openwetware.org/images/e/e1/UW_AID.jpg" height="340"/></div></html>
-</html>
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