From 2008.igem.org
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| - | <html> | + | <html>< h3> Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/ pMPM-T5-AID> pMPMT5+AID</a> construct</h3> |
| - | <p>Michał K.:</p> | + | <h4>Michał K.</h4> |
| - | <p>1. Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digestion</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/ pTrc99A-AID> pTrc99A-AID</a> with HindIII and NcoI (1x Tango buffer).</ p> | + | |
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| - | <p> 2. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols# DNA_isolation_from_agarose_gel"> isolation of DNA</a> encoding AID from proper band ( 600 bp).</ p> | + | <p> |
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| | + | <li> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols# electrotransform"> electroporation</a> with ligation ( <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5+AID</a>).</ li> |
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| - | <p>3. Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digestion</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> with HindIII and NcoI (1x Tango buffer), <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation</a> (CIAP).</p> | + | <li>Plating on LB + tetracycline.</li></ol></p> |
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| - | <p>4. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> vector from proper band (4250 bp).</p> | + | </html> |
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| - | <p>5. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments.</p>
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| - | <html><div align="center"><img src="http://openwetware.org/images/e/e1/UW_AID.jpg" height="340"/></div></html>
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Latest revision as of 16:11, 12 October 2008
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