From 2008.igem.org
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| - | <html> | + | <html><h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5+AID</a> construct</h3> |
| - | <h4>Michał K.:</h4> | + | <h4>Michał K.</h4> |
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| - | <li> Restriction <a href="https://2008.igem.org/ wiki/index.php?title=Wiki/Team:Warsaw/ protocols# digest"> digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/ vectors/pTrc99A-AID> pTrc99A-AID</a> with HindIII and NcoI ( 1x Tango buffer).</li> | + | <li> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/ igem_notebook.htm# top10"> TOP10</a> <a href= "https://2008.igem.org/Wiki/Team:Warsaw/ protocols#electrotransform"> electroporation</a> with ligation (<a href=https://2008.igem.org/Wiki/Team:Warsaw/ vectors/pMPM-T5-AID> pMPMT5+AID</a>).</li> |
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| - | <li> DNA electrophoresis and <a href= "https://2008.igem.org/Wiki/Team:Warsaw/ protocols#DNA_isolation_from_agarose_gel"> isolation of DNA</a> encoding AID from proper band (600 bp).</li> | + | |
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| - | <li> Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> with HindIII and NcoI (1x Tango buffer), <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation</a> (CIAP).</li> | + | <li>Plating on LB + tetracycline.</li></ol></p> |
| - | <li> DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> vector from proper band (4250 bp).</li>
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| - | <li>Electrophoresis of isolated DNA</li>
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| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments. </li>
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| - | </ol></p> | + | |
| - | <html><div align="center"><img src="http://openwetware.org/images/e/e1/UW_AID.jpg" height="340"/></div></html>
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Latest revision as of 16:11, 12 October 2008
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