"
Team:Warsaw/Calendar-Main/21 August 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
MKrzyszton (Talk | contribs) |
||
| Line 7: | Line 7: | ||
<h3>Cloning of protein A DNA to GeneArt plasmid</h3><h4>Antoni</h4> | <h3>Cloning of protein A DNA to GeneArt plasmid</h3><h4>Antoni</h4> | ||
<p><ol> | <p><ol> | ||
| - | <li> Colony PCR on colonies from plates with transformations pGeneart+A Primers used: | + | <li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on colonies from plates with transformations pGeneart+A Primers used: |
| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> | + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>. </li> |
| - | <li> Confirmed transformant colonies inoculated to liquid LB with ampicillin | + | <li> Confirmed transformant colonies inoculated to liquid LB with ampicillin. |
</li> | </li> | ||
| - | <li>Inoculation to liquid LB with ampicillin: pET15b+OmpA- | + | <li>Inoculation to liquid LB with ampicillin: pET15b+OmpA-alpha.</li> |
</ol></p> | </ol></p> | ||
Revision as of 16:19, 12 October 2008
 |
|
||||||||
 |
|
||||||||
|
|
