Team:Warsaw/Calendar-Main/26 May 2008

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<html><h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5+AID-T7</a></h3>
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<h4>Michał K.</h4>
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<li>  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of purified products from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/15_May_2008">15 May</a> (p.1 and p.4).</li>
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Michał K.:
 
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1. Inoculation separate transformant colonies for transcription fusion (liquid LB + tetracycline).
 
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2. Inoculation separate transformant colonies for translation fusion (liquid LB + tetracycline).  
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<li>  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electroporation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  with ligation product.</li>
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<li>  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of digested products from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/15_May_2008">15 May</a> (p.4 and p.5).</li>
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<li>  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electroporation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  with ligation product.</li>
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<li>  Transformants plating on LB + tetracycline.</li></ol>
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Latest revision as of 16:33, 12 October 2008

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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

  1. Ligation of purified products from 15 May (p.1 and p.4).
  2. Electroporation of E. coli TOP10 with ligation product.
  3. Ligation of digested products from 15 May (p.4 and p.5).
  4. Electroporation of E. coli TOP10 with ligation product.
  5. Transformants plating on LB + tetracycline.

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