Team:Warsaw/Calendar-Main/27 May 2008

From 2008.igem.org

(Difference between revisions)
 
(13 intermediate revisions not shown)
Line 1: Line 1:
{{WarNotebook}}
{{WarNotebook}}
<!-- do not edit above me! -->
<!-- do not edit above me! -->
 +
<html><h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5+AID-T7</a></h3>
 +
<h4>Michał K.</h4>
 +
<p> Further inoculation of separate transformant colonies for transcriptional and translational fusions on liquid LB + tetracycline (as there were no positive colonies in previous assays).
-
1. Isolation of hypothetical pMPM-T5 with transcription fusion
+
</p> </html>
-
 
+
-
2. Isolation of hypothetical pMPM-T5 with translation fusion
+
-
 
+
-
3. Control digest of pMPM-T5 with transcription fusion with EcoRI and XhoI
+
-
 
+
-
4. Control digest of pMPM-T5 with translation fusion with BamHI
+
-
 
+
-
5. Control digest of pMPM-T5 with translation fusion with EcoRI and XbaI
+
-
 
+
-
6. Choice of proper clones and preparation for sequencing
+
-
 
+

Latest revision as of 16:34, 12 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

Further inoculation of separate transformant colonies for transcriptional and translational fusions on liquid LB + tetracycline (as there were no positive colonies in previous assays).




Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/27_May_2008"