Team:Warsaw/Calendar-Main/27 August 2008

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<h3>Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA<br>Antoni</h3>
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<h3>Cloning of protein Z DNA to pET15b+Omp-alfa plasmid</h3><h4>Antoni</h4>
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<li> Colony PCR on colonies from plates with transformations pGeneart+A Primers used:
<li> Colony PCR on colonies from plates with transformations pGeneart+A Primers used:

Revision as of 17:23, 12 October 2008

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Cloning of protein Z DNA to pET15b+Omp-alfa plasmid

Antoni

  1. Colony PCR on colonies from plates with transformations pGeneart+A Primers used: AP+NotI and AL+SacI
  2. Confirmed transformant colonies inoculated to liquid LB with ampicillin



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