Team:Warsaw/Calendar-Main/28 August 2008

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<h3>Cloning of protein Z DNA to pET15b+Omp-alfa plasmid</h3><h4>Antoni</h4>
<h3>Cloning of protein Z DNA to pET15b+Omp-alfa plasmid</h3><h4>Antoni</h4>
<p><ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pET15b+OmpA-alfa plasmids.</li>
<p><ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pET15b+OmpA-alfa plasmids.</li>
 +
<li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of pGeneart+A plasmids with NdeI and NotI (Orange buffer). </li>
 +
<li>Gel elctrophoresis. Chioce of proper clone. </li>
</ol></p>
</ol></p>

Revision as of 17:55, 12 October 2008

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Cloning of protein Z DNA to pET15b+Omp-alfa plasmid

Antoni

  1. Isolation of pET15b+OmpA-alfa plasmids.
  2. Control digest of pGeneart+A plasmids with NdeI and NotI (Orange buffer).
  3. Gel elctrophoresis. Chioce of proper clone.

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