Team:Warsaw/Calendar-Main/30 June 2008

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<h3>Cloning alpha-A and omega-A fusions on pKS</h3>
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<h3>Cloning alpha-A and omega-A fusions on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a></h3>
<h4>Michał L., Ewa, Marcin</h4>
<h4>Michał L., Ewa, Marcin</h4>
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<h3>Preparation of constructs with OmpA protein fusions</h3><h4>Paweł</h4>
<h3>Preparation of constructs with OmpA protein fusions</h3><h4>Paweł</h4>
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<p> Inoculation of <i> E. coli</i> with pET15b plasmid on LB + ampicillin.</p>
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<p> Inoculation of <i> E. coli</i> with ~<A href=http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf>pET15b</a> plasmid on LB + ampicillin.</p>
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Revision as of 21:56, 12 October 2008

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Cloning alpha-A and omega-A fusions on pKS

Michał L., Ewa, Marcin

Inoculation of liquid LB medium with 6 transformant colonies.

Preparation of constructs with OmpA protein fusions

Paweł

Inoculation of E. coli with ~pET15b plasmid on LB + ampicillin.