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Team:Warsaw/Calendar-Main/28 August 2008
From 2008.igem.org
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| - | <h3>Cloning of protein Z DNA to pET15b+Omp- | + | <h3>Cloning of protein Z DNA to pET15b+Omp-alpha plasmid</h3><h4>Antoni</h4> |
<p><ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pET15b+OmpA-alfa plasmids.</li> | <p><ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pET15b+OmpA-alfa plasmids.</li> | ||
<li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of pGeneart+A plasmids with NdeI and NotI (Orange buffer). </li> | <li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of pGeneart+A plasmids with NdeI and NotI (Orange buffer). </li> | ||
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| - | <h3>Cloning of protein A DNA to pET15b+Omp- | + | <h3>Cloning of protein A DNA to pET15b+Omp-alpha plasmid</h3><h4>Michał K.</h4> |
| + | <ol><li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on colonies from plates with transformations pEt15b+A_alpha. <br>Primers used: | ||
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>. </li> | ||
| + | <li>Gel electrophoresis.</li> | ||
| + | <li> Confirmed transformant colonies inoculated to liquid LB with ampicillin. | ||
| + | </li></ol> | ||
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Cloning of protein Z DNA to pET15b+Omp-alpha plasmidAntoni
Cloning of protein A DNA to pET15b+Omp-alpha plasmidMichał K.
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