Team:Warsaw/Calendar-Main/28 August 2008

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<h3>Cloning of protein A DNA to pET15b+Omp-alpha plasmid</h3><h4>Michał K.</h4>
<h3>Cloning of protein A DNA to pET15b+Omp-alpha plasmid</h3><h4>Michał K.</h4>
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<ol><li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on colonies from plates with transformations pEt15b+A_alpha. <br>Primers used:
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<ol><li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on colonies from plates with transformation pEt15b+A_alpha. <br>Primers used:
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>. </li>  
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>. </li>  

Revision as of 22:02, 12 October 2008

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Cloning of protein Z DNA to pET15b+Omp-alpha plasmid

Antoni

  1. Isolation of pET15b+OmpA-alpha plasmids.
  2. Control digest of pGeneart+A plasmids with NdeI and NotI (Orange buffer).
  3. Gel elctrophoresis. Chioce of proper clone.

Cloning of protein A DNA to pET15b+Omp-alpha plasmid

Michał K.

  1. Colony PCR on colonies from plates with transformation pEt15b+A_alpha.
    Primers used: AP+NotI and AL+SacI.
  2. Gel electrophoresis.
  3. Confirmed transformant colonies inoculated to liquid LB with ampicillin.

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