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Team:Warsaw/Calendar-Main/29 August 2008
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| + | <h3>Cloning of protein A DNA to pET15b+Omp-alpha plasmid</h3><h4>Michał K.</h4> | ||
| + | <p><ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pET15b+A-alpha plasmids.</li> | ||
| + | <li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with NdeI and NotI (Orange buffer). </li> | ||
| + | <li>Gel elctrophoresis. Chioce of proper clone. </li> | ||
| + | </ol></p> | ||
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<h3>Protein purification: A-alpha, Z-alpha i Z-omega</h3> | <h3>Protein purification: A-alpha, Z-alpha i Z-omega</h3> | ||
<h4>Piotr</h4> | <h4>Piotr</h4> | ||
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| - | Single transformations of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> with plasmids pET15b+A-alfa, pET15b+Z-alfa and pET15b+Z-omega. Plating them on LB with | + | Single transformations of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> with plasmids pET15b+A-alfa, pET15b+Z-alfa and pET15b+Z-omega. Plating them on LB with chloramphenicol and ampicillin.</p></html> |
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Cloning of protein A DNA to pET15b+Omp-alpha plasmidMichał K.
Protein purification: A-alpha, Z-alpha i Z-omegaPiotrSingle transformations of Rosetta with plasmids pET15b+A-alfa, pET15b+Z-alfa and pET15b+Z-omega. Plating them on LB with chloramphenicol and ampicillin.
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