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Team:Warsaw/Calendar-Main/3 July 2008
From 2008.igem.org
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<h3>Preparation of constructs with OmpA protein fusions</h3> | <h3>Preparation of constructs with OmpA protein fusions</h3> | ||
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Colonies of each transformants from previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA_alpha</a> and https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b+OmpA_omega</a>) were inoculated to liquid LB + ampicillin.</p> | Colonies of each transformants from previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA_alpha</a> and https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b+OmpA_omega</a>) were inoculated to liquid LB + ampicillin.</p> | ||
| - | + | <h3>Cloning alpha-A and omega-A fusions on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a></h3> | |
| - | <h3>Cloning alpha-A and omega-A fusions on pKS</h3> | + | |
<h4>Michał L., Ewa, Marcin</h4> | <h4>Michał L., Ewa, Marcin</h4> | ||
<p>We have received sequencing results A-alpha fusion sequence is OK but in case of Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 primer which causes frameshift and cloning of this fusion has to be repeated from the beginning.</p> | <p>We have received sequencing results A-alpha fusion sequence is OK but in case of Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 primer which causes frameshift and cloning of this fusion has to be repeated from the beginning.</p> | ||
Revision as of 22:55, 12 October 2008
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Preparation of constructs with OmpA protein fusionsPawełColonies of each transformants from previous day (pET15b+OmpA_alpha and https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin. Cloning alpha-A and omega-A fusions on pKSMichał L., Ewa, MarcinWe have received sequencing results A-alpha fusion sequence is OK but in case of Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 primer which causes frameshift and cloning of this fusion has to be repeated from the beginning.
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