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Team:Warsaw/Calendar-Main/25 September 2008
From 2008.igem.org
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| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on alpha_linker and linker_A with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL+SacI">AlphaL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> primers and 10% DMSO (30 cycles, elongation | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on alpha_linker and linker_A with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL+SacI">AlphaL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> primers and 10% DMSO (30 cycles, elongation 60 s, annealing temperature 72°C). </li> |
<li> Gel electrophoresis of PCR products and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band (1019 bp).</li> | <li> Gel electrophoresis of PCR products and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band (1019 bp).</li> | ||
<li>Measurment of concentration of both isolated products.</li> | <li>Measurment of concentration of both isolated products.</li> | ||
Revision as of 23:15, 13 October 2008
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Mutagenesis of protein APawełTreatment of mutageneses as on 23rd September. Preparation of alpha_A constructAntoni
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