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Team:Warsaw/Calendar-Main/18 July 2008
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MKrzyszton (Talk | contribs) (New page: {{WarNotebook}} <!-- do not edit above me! --> <p>'''Cloning of protein A DNA to OmpA constructs'''<br> </p> <!-- do not remove this! --> {{WarNotebookEnd}}) |
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| - | < | + | <h3>Cloning of protein Z DNA to <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b-OmpA-omega</a> in place of OmpA</h3><h4>Paweł</h4> |
| - | </p> | + | <p>Ligation <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformed</a> into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain and plated on LB + ampicillin.</p> |
| + | <h3>Cloning of protein Z DNA to OmpA constructs</h3> | ||
| + | <h4>Michał K.</h4> | ||
| + | <p>Colonies from transformation <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> were inoculated to liquid LB broth with kanamycin. </p> | ||
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Latest revision as of 09:52, 14 October 2008
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Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpAPawełLigation transformed into TOP10 strain and plated on LB + ampicillin. Cloning of protein Z DNA to OmpA constructsMichał K.Colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin. |
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