Team:Warsaw/Calendar-Main/18 July 2008

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Latest revision as of 09:52, 14 October 2008


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Ligation transformed into TOP10 strain and plated on LB + ampicillin.

Colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin.

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-<p>'''Cloning of protein A DNA to OmpA constructs'''<br>
+<h3>Cloning of protein Z DNA to <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b-OmpA-omega</a> in place of OmpA</h3><h4>Paweł</h4>
-. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega and pACYC177+OmpA_A_alpha. Primers used:
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+<p>Ligation <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformed</a> into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain and plated on LB + ampicillin.</p>
-<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>
-</html><br>
-. Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate) inoculated to liquid LB with kanamycin. <br>
+<h3>Cloning of protein Z DNA to OmpA constructs</h3>
-</p>
+<h4>Michał K.</h4>
+<p>Colonies from transformation <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> were inoculated to liquid LB broth with kanamycin. </p>
+<br>
-<p>Paweł: pET15b vector purified.
-Digested of pET15b and pACYClac+OmpA+omega with BamHI and NdeI</p>
+</html>
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