Team:Warsaw/Calendar-Main/18 July 2008

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<h3>Cloning of protein A DNA to OmpA constructs</h3>
 
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<li>Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega and pACYC177+OmpA_A_alpha. Primers used:
 
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>
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<h3>Cloning of protein Z DNA to <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b-OmpA-omega</a> in place of OmpA</h3><h4>Paweł</h4>
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<li> Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate) inoculated to liquid LB with kanamycin. </li></ol>
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<p>Ligation <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformed</a> into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain and plated on LB + ampicillin.</p>
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<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
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Paweł</h3>
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<p><ol><li>Ligation <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformed</a> into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain and plated on LB+kanamycin</li>
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<h3>Cloning of protein Z DNA to OmpA constructs</h3>
<h3>Cloning of protein Z DNA to OmpA constructs</h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
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<p><ol><li> 4 colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin </li></ol></p>
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<p>Colonies from transformation <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> were inoculated to liquid LB broth with kanamycin. </p>
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Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA

Paweł

Ligation transformed into TOP10 strain and plated on LB + ampicillin.

Cloning of protein Z DNA to OmpA constructs

Michał K.

Colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin.


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