Team:Warsaw/Calendar-Main/19 July 2008

From 2008.igem.org

(Difference between revisions)
(New page: {{WarNotebook}} <!-- do not edit above me! --> <p>'''Cloning of protein A DNA to OmpA constructs''' <br> </p> <!-- do not remove this! --> {{WarNotebookEnd}})
 
(14 intermediate revisions not shown)
Line 2: Line 2:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
 +
<html>
 +
<h3>Cloning of protein Z DNA to OmpA constructs</h3>
 +
<h4>Michał K.</h4>
 +
<p><ol>
 +
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a>). </li>
 +
<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer).</li> <li> Gel electrophoresis - we found 4 good clones.</li></ol></p>
-
<p>'''Cloning of protein A DNA to OmpA constructs''' <br>
 
-
</p>
 
 +
 +
 +
 +
</html>

Latest revision as of 09:53, 14 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Cloning of protein Z DNA to OmpA constructs

Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha).
  2. Control digest of isolated plasmids with BamHI and SacI (BamHI buffer).
  3. Gel electrophoresis - we found 4 good clones.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/19_July_2008"