Team:Warsaw/Calendar-Main/19 July 2008

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<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
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<h3>Cloning of protein Z DNA to OmpA constructs</h3>
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Paweł</h3>
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<h4>Michał K.</h4>
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<p><ol><li>Result of transformation of pET15b+omega with protein Z DNA: 4 colonies grown</li>
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<li>Each colony cultured overnight in LB+amp</li>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a>). </li>
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer).</li> <li> Gel electrophoresis - we found 4 good clones.</li></ol></p>
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Latest revision as of 09:53, 14 October 2008

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Cloning of protein Z DNA to OmpA constructs

Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha).
  2. Control digest of isolated plasmids with BamHI and SacI (BamHI buffer).
  3. Gel electrophoresis - we found 4 good clones.

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