Team:Warsaw/Calendar-Main/19 July 2008

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Latest revision as of 09:53, 14 October 2008


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Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha).
Control digest of isolated plasmids with BamHI and SacI (BamHI buffer).
Gel electrophoresis - we found 4 good clones.

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-<h3>Cloning of protein Z DNA to OmpA constructs<br>Michał K.</h3>
+<h3>Cloning of protein Z DNA to OmpA constructs</h3>
+<h4>Michał K.</h4>
 <p><ol>
-<li> Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha). </li>
+<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a>). </li>
-<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI (we found 4 good clones).</li></ol></p>
+<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer).</li> <li> Gel electrophoresis - we found 4 good clones.</li></ol></p>
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-<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
-Paweł</h3>
-<p><ol><li>Result of transformation of pET15b+omega with protein Z DNA: 4 colonies grown</li>
-<li>Each colony cultured overnight in LB+amp</li>
-</ol>
-</p>
-<h3> Michał L., Ewa, Marcin</h3>
-We have finally got rid of phage infection and we are able to work with E. coli again. Hurray!
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