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-	==[[User:Sixpi|Sixpi]] 22:25, 4 June 2008 (UTC)==	+	__TOC__
		+
		+	==pBca1256-K112000==
	<pre>		<pre>
-	PCR bx1/bx2 on Bjh1301         (688 bp, EcoRI/BamHI/DpnI)	+	PCR bx1/bx2 on Bjh1301 (688 bp, EcoRI/BamHI/DpnI)
	Sub into pBca1256 (EcoRI/BamHI)		Sub into pBca1256 (EcoRI/BamHI)
	Product is pBca1256-K112000		Product is pBca1256-K112000
	----------------------------------------		----------------------------------------
	bx1 Forward Biobricking of {T4 Holin!}		bx1 Forward Biobricking of {T4 Holin!}
-	CCATAGAATTCatgAGATCTatggcagcacctagaatatcat	+	ccagtGAATTCatgAGATCTatggcagcacctagaatatc
	bx2 Reverse Biobricking of {T4 Holin!}		bx2 Reverse Biobricking of {T4 Holin!}
-	cgttaGGATCCttatttagcccttcctaatattct	+	cgttaGGATCCttatttagcccttcctaatattctggccgcc
	</pre>		</pre>
		+	==pBca1256-K112001==
	<pre>		<pre>
-	PCR bx3/bx2 on Bjh1301         (685 bp, EcoRI/BamHI/DpnI)	+	PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
	Sub into pBca1256 (EcoRI/BamHI)		Sub into pBca1256 (EcoRI/BamHI)
	Product is pBca1256-K112001		Product is pBca1256-K112001
	----------------------------------------		----------------------------------------
	bx3 Forward biobricking of {<T4 Holin!}		bx3 Forward biobricking of {<T4 Holin!}
-	CCATAGAATTCatgAGATCTgcagcacctagaatatcattttcg	+	ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
	bx2 Reverse biobricking of {<T4 Holin!}		bx2 Reverse biobricking of {<T4 Holin!}
-	cgttaGGATCCttatttagcccttcctaatattc	+	cgttaGGATCCttatttagcccttcctaatattctggccgcc
	</pre>		</pre>
		+	==pBca1256-K112002==
	<pre>		<pre>
-	PCR bx1/bx4 on Bjh1301         (685 bp, EcoRI/BamHI/DpnI)	+	PCR bx1/bx4 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
	Sub into pBca1256 (EcoRI/BamHI)		Sub into pBca1256 (EcoRI/BamHI)
	Product is pBca1256-K112002		Product is pBca1256-K112002
	----------------------------------------		----------------------------------------
	bx1 Forward biobricking of {T4 Holin>}		bx1 Forward biobricking of {T4 Holin>}
-	CCATAGAATTCatgAGATCTatggcagcacctagaatatcat	+	ccagtGAATTCatgAGATCTatggcagcacctagaatatc
	bx4 Reverse biobricking of {T4 Holin>}		bx4 Reverse biobricking of {T4 Holin>}
	cgttaGGATCCtttagcccttcctaatattctggcc		cgttaGGATCCtttagcccttcctaatattctggcc
	</pre>		</pre>
		+	==pBca1256-K112003==
	<pre>		<pre>
-	PCR bx3/bx4 on Bjh1301         (682 bp, EcoRI/BamHI/DpnI)	+	PCR bx3/bx4 on Bjh1301 (682 bp, EcoRI/BamHI/DpnI)
	Sub into pBca1256 (EcoRI/BamHI)		Sub into pBca1256 (EcoRI/BamHI)
	Product is pBca1256-K112003		Product is pBca1256-K112003
	----------------------------------------		----------------------------------------
	bx3 Forward Biobricking of {<T4 Holin>}		bx3 Forward Biobricking of {<T4 Holin>}
-	CCATAGAATTCatgAGATCTgcagcacctagaatatcattttcg	+	ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
	bx4 Reverse Biobricking of {<T4 Holin>}		bx4 Reverse Biobricking of {<T4 Holin>}
	cgttaGGATCCtttagcccttcctaatattctggcc		cgttaGGATCCtttagcccttcctaatattctggcc
	</pre>		</pre>
		+
		+	==pBca1256-K112004==
		+	<pre>
		+	PCR bx5/bx2 on Bjh1301 (692 bp, EcoRI/BamHI/DpnI)
		+	Sub into pBca1256 (EcoRI/BamHI)
		+	Product is pBca1256-K112004
		+	----------------------------------------
		+	bx5 Forward Biobricking of {a~T4 Holin!}
		+	ccagtGAATTCatgAGATCTgtctatggcagcacctagaatatc
		+	bx2 Reverse Biobricking of {a~T4 Holin!}
		+	cgttaGGATCCttatttagcccttcctaatattctggccgcc
		+	</pre>
		+
		+	==pBca1256-K112005==
		+	<pre>
		+	PCR bx6/bx2 on Bjh1301 (704 bp, EcoRI/BamHI/DpnI)
		+	Sub into pBca1256 (EcoRI/BamHI)
		+	Product is pBca1256-K112005
		+	----------------------------------------
		+	bx6 Forward Biobricking of {rbs.T4 Holin!}
		+	ccagtGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc
		+	bx2 Reverse Biobricking of {rbs.T4 Holin!}
		+	cgttaGGATCCttatttagcccttcctaatattctggccgcc
		+	</pre>
		+
		+	==pBca1256-K112006==
		+	<pre>
		+	PCR bx7/bx8 on Bjh1303 (325 bp, EcoRI/BamHI/DpnI)
		+	Sub into pBca1256 (EcoRI/BamHI)
		+	Product is pBca1256-K112006
		+	----------------------------------------
		+	bx7 Forward biobricking of {T4 Antiholin!}
		+	ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
		+	bx8 Reverse biobricking of {T4 Antiholin!}
		+	cgttaGGATCCtcattcagtctccaatttaatgttc
		+	</pre>
		+
		+	==pBca1256-K112007==
		+	<pre>
		+	PCR bx9/bx8 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
		+	Sub into pBca1256 (EcoRI/BamHI)
		+	Product is pBca1256-K112007
		+	----------------------------------------
		+	bx9 Forward biobricking of {<T4 Antiholin!}
		+	ccagtGAATTCatgAGATCTgccttaaaagcaacagcac
		+	bx8 Reverse biobricking of {<T4 Antiholin!}
		+	cgttaGGATCCtcattcagtctccaatttaatgttc
		+	</pre>
		+
		+	==pBca1256-K112008==
		+	<pre>
		+	PCR bx7/bx10 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
		+	Sub into pBca1256 (EcoRI/BamHI)
		+	Product is pBca1256-K112008
		+	----------------------------------------
		+	bx7 Forward biobricking of {T4 Antiholin>}
		+	ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
		+	bx10 Reverse biobricking of {T4 Antiholin>}
		+	cgttaGGATCCttcagtctccaatttaatgttc
		+	</pre>
		+
		+	==pBca1256-K112009==
		+	<pre>
		+	PCR bx9/bx10 on Bjh1303 (319 bp, EcoRI/BamHI/DpnI)
		+	Sub into pBca1256 (EcoRI/BamHI)
		+	Product is pBca1256-K112009
		+	----------------------------------------
		+	bx9 Forward biobricking of {<T4 Antiholin>}
		+	ccagtGAATTCatgAGATCTgccttaaaagcaacagcac
		+	bx10 Reverse biobricking of {<T4 Antiholin>}
		+	cgttaGGATCCttcagtctccaatttaatgttc
		+	</pre>
		+
		+	==pBca1256-K112010==
		+	<pre>
		+	PCR bx11/bx8 on Bjh1303 (329 bp, EcoRI/BamHI/DpnI)
		+	Sub into pBca1256 (EcoRI/BamHI)
		+	Product is pBca1256-K112010
		+	----------------------------------------
		+	bx11 Forward biobricking of {a~T4 Antiholin!}
		+	ccagtGAATTCatgAGATCTctttatggccttaaaagcaacagc
		+	bx8 Reverse biobricking of {a~T4 Antiholin!}
		+	cgttaGGATCCtcattcagtctccaatttaatgttc
		+	</pre>
		+
		+	==pBca1256-K112011==
		+	<pre>
		+	PCR bx12/bx8 on Bjh1303 (339 bp, EcoRI/BamHI/DpnI)
		+	Sub into pBca1256 (EcoRI/BamHI)
		+	Product is pBca1256-K112011
		+	----------------------------------------
		+	bx12 Forward biobricking of {rbs.T4 Antiholin!}
		+	ccagtGAATTCatgAGATCTgataggaggcctttatggccttaaaagcaacagc
		+	bx8 Reverse biobricking of {rbs.T4 Antiholin!}
		+	cgttaGGATCCtcattcagtctccaatttaatgttc
		+	</pre>
		+
		+	==pBca1256-K112012==
		+	<pre>
		+	PCR bx13/bx14 on Bjh1302 (526 bp, EcoRI/BamHI/DpnI)
		+	Sub into pBca1256 (EcoRI/BamHI)
		+	Product is pBca1256-K112012
		+	----------------------------------------
		+	bx13      Forward biobricking of {T4 Endolysin!}
		+	ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg
		+	bx14      Reverse biobricking of {T4 Endolysin!}
		+	cgttaGGATCCttatagatttttatacgcgtcccaagtgcc
		+	</pre>
		+
		+	==pBca1256-K112013==
		+	<pre>
		+	PCR bx15/bx14 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI)
		+	Sub into pBca1256 (EcoRI/BamHI)
		+	Product is pBca1256-K112013
		+	----------------------------------------
		+	bx15      Forward biobricking of {<T4 Endolysin!}
		+	ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg
		+	bx14      Reverse biobricking of {<T4 Endolysin!}
		+	cgttaGGATCCttatagatttttatacgcgtcccaagtgcc
		+	</pre>
		+
		+	==pBca1256-K112014==
		+	<pre>
		+	PCR bx13/bx16 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI)
		+	Sub into pBca1256 (EcoRI/BamHI)
		+	Product is pBca1256-K112014
		+	----------------------------------------
		+	bx13      Forward biobricking of {T4 Endolysin>}
		+	ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg
		+	bx16      Reverse biobricking of {T4 Endolysin>}
		+	cgttaGGATCCtagatttttatacgcgtcccaagtgcc
		+	</pre>
		+
		+	==pBca1256-K112015==
		+	<pre>
		+	PCR bx15/bx16 on Bjh1302 (520 bp, EcoRI/BamHI/DpnI)
		+	Sub into pBca1256 (EcoRI/BamHI)
		+	Product is pBca1256-K112015
		+	----------------------------------------
		+	bx15      Forward biobricking of {<T4 Endolysin>}
		+	ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg
		+	bx16      Reverse biobricking of {<T4 Endolysin>}
		+	cgttaGGATCCtagatttttatacgcgtcccaagtgcc
		+	</pre>
		+
		+	==pBca1256-K112016==
		+	<pre>
		+	PCR bx17/bx16 on Bjh1302 (530 bp, EcoRI/BamHI/DpnI)
		+	Sub into pBca1256 (EcoRI/BamHI)
		+	Product is pBca1256-K112016
		+	----------------------------------------
		+	bx17      Forward biobricking of {a~T4 Endolysin!}
		+	ccagtGAATTCatgAGATCTtattatgaatatatttgaaatgttacgtatagatg
		+	bx16      Reverse biobricking of {a~T4 Endolysin!}
		+	cgttaGGATCCtagatttttatacgcgtcccaagtgcc
		+	</pre>
		+
		+	==pBca1256-K112017==
		+	<pre>
		+	PCR bx18/bx16 on Bjh1302 (542 bp, EcoRI/BamHI/DpnI)
		+	Sub into pBca1256 (EcoRI/BamHI)
		+	Product is pBca1256-K112017
		+	----------------------------------------
		+	bx18      Forward biobricking of {rbs.T4 Endolysin!}
		+	ccagtGAATTCatgAGATCTatacttaggaggtattatgaatatatttgaaatgttac
		+	bx16      Reverse biobricking of {rbs.T4 Endolysin!}
		+	cgttaGGATCCtagatttttatacgcgtcccaagtgcc
		+	</pre>
		+
-	[[Team:UC_Berkeley/Notebook/Bing_Xia|Bing Xia]]<br>	+	----
		+	<div style="text-align: center;"> [[Team:UC_Berkeley/Notebook/Bing_Xia|Bing Xia]] </div>
		+	<div style="text-align: center;"> [[Team:UC_Berkeley|Back to Berkeley]] </div>

---

Latest revision as of 01:36, 12 June 2008

Contents 1 pBca1256-K112000 2 pBca1256-K112001 3 pBca1256-K112002 4 pBca1256-K112003 5 pBca1256-K112004 6 pBca1256-K112005 7 pBca1256-K112006 8 pBca1256-K112007 9 pBca1256-K112008 10 pBca1256-K112009 11 pBca1256-K112010 12 pBca1256-K112011 13 pBca1256-K112012 14 pBca1256-K112013 15 pBca1256-K112014 16 pBca1256-K112015 17 pBca1256-K112016 18 pBca1256-K112017

pBca1256-K112000

PCR bx1/bx2 on Bjh1301		(688 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112000
----------------------------------------
bx1		Forward Biobricking of {T4 Holin!}
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx2		Reverse Biobricking of {T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112001

PCR bx3/bx2 on Bjh1301		(685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112001
----------------------------------------
bx3		Forward biobricking of {<T4 Holin!}
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx2		Reverse biobricking of {<T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112002

PCR bx1/bx4 on Bjh1301		(685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112002
----------------------------------------
bx1		Forward biobricking of {T4 Holin>}
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx4		Reverse biobricking of {T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc

pBca1256-K112003

PCR bx3/bx4 on Bjh1301		(682 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112003
----------------------------------------
bx3		Forward Biobricking of {<T4 Holin>}
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx4		Reverse Biobricking of {<T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc

pBca1256-K112004

PCR bx5/bx2 on Bjh1301		(692 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112004
----------------------------------------
bx5		Forward Biobricking of {a~T4 Holin!}
ccagtGAATTCatgAGATCTgtctatggcagcacctagaatatc
bx2		Reverse Biobricking of {a~T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112005

PCR bx6/bx2 on Bjh1301		(704 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112005
----------------------------------------
bx6		Forward Biobricking of {rbs.T4 Holin!}
ccagtGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc
bx2		Reverse Biobricking of {rbs.T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112006

PCR bx7/bx8 on Bjh1303		(325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112006
----------------------------------------
bx7		Forward biobricking of {T4 Antiholin!}
ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
bx8		Reverse biobricking of {T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112007

PCR bx9/bx8 on Bjh1303		(322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112007
----------------------------------------
bx9		Forward biobricking of {<T4 Antiholin!}
ccagtGAATTCatgAGATCTgccttaaaagcaacagcac
bx8		Reverse biobricking of {<T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112008

PCR bx7/bx10 on Bjh1303		(322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112008
----------------------------------------
bx7		Forward biobricking of {T4 Antiholin>}
ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
bx10	Reverse biobricking of {T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc

pBca1256-K112009

PCR bx9/bx10 on Bjh1303		(319 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112009
----------------------------------------
bx9		Forward biobricking of {<T4 Antiholin>}
ccagtGAATTCatgAGATCTgccttaaaagcaacagcac
bx10	Reverse biobricking of {<T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc

pBca1256-K112010

PCR bx11/bx8 on Bjh1303		(329 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112010
----------------------------------------
bx11	Forward biobricking of {a~T4 Antiholin!}
ccagtGAATTCatgAGATCTctttatggccttaaaagcaacagc
bx8		Reverse biobricking of {a~T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112011

PCR bx12/bx8 on Bjh1303		(339 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112011
----------------------------------------
bx12	Forward biobricking of {rbs.T4 Antiholin!}
ccagtGAATTCatgAGATCTgataggaggcctttatggccttaaaagcaacagc
bx8		Reverse biobricking of {rbs.T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112012

PCR bx13/bx14 on Bjh1302		(526 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112012
----------------------------------------
bx13      Forward biobricking of {T4 Endolysin!}
ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg
bx14      Reverse biobricking of {T4 Endolysin!}
cgttaGGATCCttatagatttttatacgcgtcccaagtgcc

pBca1256-K112013

PCR bx15/bx14 on Bjh1302		(523 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112013
----------------------------------------
bx15      Forward biobricking of {<T4 Endolysin!}
ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg
bx14      Reverse biobricking of {<T4 Endolysin!}
cgttaGGATCCttatagatttttatacgcgtcccaagtgcc

pBca1256-K112014

PCR bx13/bx16 on Bjh1302		(523 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112014
----------------------------------------
bx13      Forward biobricking of {T4 Endolysin>}
ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg
bx16      Reverse biobricking of {T4 Endolysin>}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc

pBca1256-K112015

PCR bx15/bx16 on Bjh1302		(520 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112015
----------------------------------------
bx15      Forward biobricking of {<T4 Endolysin>}
ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg
bx16      Reverse biobricking of {<T4 Endolysin>}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc

pBca1256-K112016

PCR bx17/bx16 on Bjh1302		(530 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112016
----------------------------------------
bx17      Forward biobricking of {a~T4 Endolysin!}
ccagtGAATTCatgAGATCTtattatgaatatatttgaaatgttacgtatagatg
bx16      Reverse biobricking of {a~T4 Endolysin!}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc

pBca1256-K112017

PCR bx18/bx16 on Bjh1302		(542 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112017
----------------------------------------
bx18      Forward biobricking of {rbs.T4 Endolysin!}
ccagtGAATTCatgAGATCTatacttaggaggtattatgaatatatttgaaatgttac
bx16      Reverse biobricking of {rbs.T4 Endolysin!}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc

---

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