Team:Warsaw/Calendar-Main/17 September 2008

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<h3>Optimisation of primers for preparation of parts</h3>
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<h4>Michał K.</h4>
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<ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pACYC177+OmpA_omega_deltaA_alpha plasmid using
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL_BNXNE">AL_BNXNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+XhoI+SpeI">AP+XhoI+SpeI</a>
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primers (elongation length 45s). </li>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pACYC177+OmpA_omega_deltaA_alpha plasmid using
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinLSXNE">LinLSXNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlfaPSpe">AlfaPSpe</a>
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primers (elongation length 45s). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008#fig2">Fig. 2</a> and <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008#fig3">Fig. 3</a>.</li>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on <a href=http://www.fermentas.com/techinfo/nucleicacids/mappuc1819.htm>pUC19</a> plasmid using
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">OmegaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a>
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primers (elongation length 30s). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008#fig3">Fig. 3</a> and <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008#fig4">Fig. 4</a>.</li>
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<p>Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees.</p>

Revision as of 20:54, 15 October 2008

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Testy na konkurencje Piotr Izolacja plazmidów z chodowli z poprzedniego dnia, cięcie saci i bamhi, elektroforeza

(zdjęcie żelu)

komórki mające białko oddziałujące wygrywają konkurencję!!!


Testy na MutD5: Piotr

zaszczepienie MutD5 do pożywki z Tc


Optimisation of primers for preparation of parts

Michał K.

  1. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pACYC177+OmpA_omega_deltaA_alpha plasmid using <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL_BNXNE">AL_BNXNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+XhoI+SpeI">AP+XhoI+SpeI</a> primers (elongation length 45s).
  2. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pACYC177+OmpA_omega_deltaA_alpha plasmid using <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinLSXNE">LinLSXNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlfaPSpe">AlfaPSpe</a> primers (elongation length 45s). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008#fig2">Fig. 2</a> and <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008#fig3">Fig. 3</a>.
  3. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on <a href=http://www.fermentas.com/techinfo/nucleicacids/mappuc1819.htm>pUC19</a> plasmid using <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">OmegaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a> primers (elongation length 30s). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008#fig3">Fig. 3</a> and <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008#fig4">Fig. 4</a>.

    4. Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees.
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