Team:Warsaw/Calendar-Main/25 June 2008

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<li>We have successfully amplified Alpha-link and link-A. Now it's time for PCL (Polymerase Chain Ligation) to create fusions  Alpha-A and Omega-A (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/25_June_2008#fig1">Fig. 1</a>).</li></ol>
<li>We have successfully amplified Alpha-link and link-A. Now it's time for PCL (Polymerase Chain Ligation) to create fusions  Alpha-A and Omega-A (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/25_June_2008#fig1">Fig. 1</a>).</li></ol>
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<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/8/89/PCR_gradient_Alinker_WAW.jpg" width=400/></a>
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<var> <b>Fig. 1.</b> PCR product - link-A.</var>
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<h3>Change of the reporter from pZC with B-galactosidaze to GFP or RFP</h3>
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<h4>Piotr, Weronika</h4>
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<ol>
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<li>Inoculation 10ml LB with E.coli TOP10 carrying pZC</li>
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<li>Isolation and chemotransformation with plasmids nazwa (Gfp genetrator), nazwa (RFP generator)</li></ol>
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/8/89/PCR_gradient_Alinker_WAW.jpg" width=400/></a>
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/8/89/PCR_gradient_Alinker_WAW.jpg" width=400/></a>

Revision as of 08:37, 16 October 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

Repetition of PCRs and gel-out purifications from 16 June.

PCR of Alpha-link and link-A

Michał L. Marcin and Ewa

  1. We've run gradient PCR to obtain Alpha-link and link-A.
    Annealing temp 48°C - 55°C.
    Elongation time 1 - 2 minutes.
  2. We have successfully amplified Alpha-link and link-A. Now it's time for PCL (Polymerase Chain Ligation) to create fusions Alpha-A and Omega-A (Fig. 1).
Fig. 1. PCR product - link-A.

Change of the reporter from pZC with B-galactosidaze to GFP or RFP

Piotr, Weronika

  1. Inoculation 10ml LB with E.coli TOP10 carrying pZC
  2. Isolation and chemotransformation with plasmids nazwa (Gfp genetrator), nazwa (RFP generator)
Fig. 1. PCR product - link-A.

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