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Wisconsin: Lignin Project/16 June 2008
From 2008.igem.org
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{|align="justify" style="color:#aada84;background-color:#000;" width="800 px" | {|align="justify" style="color:#aada84;background-color:#000;" width="800 px" | ||
| - | |'''Team Sorbitol''' | + | |'''Team Sorbitol:''' |
Harvested the pBAD30 and pBAD18 from the cells using a Qiagen Miniprep Kit. | Harvested the pBAD30 and pBAD18 from the cells using a Qiagen Miniprep Kit. | ||
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Began cultures of MG1655 to use for cloning. | Began cultures of MG1655 to use for cloning. | ||
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| + | Experimented with different media recipes for optimal growth. | ||
| + | M9 minimal media with: 1.) 20%glucose +++, 2.) 20% glycerol++, 3.) 20%acetate+ | ||
| + | |||
| + | Met with Jennie Reed, modeling work adding sorbitol to basic cell model | ||
'''Team Fungus:'''<br> | '''Team Fungus:'''<br> | ||
Latest revision as of 16:35, 18 October 2008
| Team Sorbitol:
Harvested the pBAD30 and pBAD18 from the cells using a Qiagen Miniprep Kit. Stored this DNA in a -20 Freezer Began cultures of MG1655 to use for cloning. Experimented with different media recipes for optimal growth. M9 minimal media with: 1.) 20%glucose +++, 2.) 20% glycerol++, 3.) 20%acetate+ Met with Jennie Reed, modeling work adding sorbitol to basic cell model Team Fungus: |
