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Team:Warsaw/Calendar-Main/17 September 2008
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pACYC177+OmpA_omega_deltaA_alpha plasmid using | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pACYC177+OmpA_omega_deltaA_alpha plasmid using | ||
| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmLNXNEB">OmLNXNEB</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers# | + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmLNXNEB">OmLNXNEB</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinPBSNP">LinPBSNP</a> |
primers (elongation length 90s). </li> | primers (elongation length 90s). </li> | ||
<p>Each reaction was carried out with 25 cycles and in temperature gradient from 55 to 75 °C.</p> | <p>Each reaction was carried out with 25 cycles and in temperature gradient from 55 to 75 °C.</p> | ||
Revision as of 16:56, 18 October 2008
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Competition testsPiotr
Conclusion: cells with interracting protein survive competition! MutD5 testingEmiliaInoculation of MutD5 into medium with tetracycline. Optimisation of primers for preparation of partsMichał K.
Each reaction was carried out with 25 cycles and in temperature gradient from 55 to 75 °C.
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