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Wisconsin: Lignin Project/24 June 2008
From 2008.igem.org
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| + | |'''Team Sorbitol:''' | ||
| + | Attempted to isolate genomic DNA from E. coli MG1655 using the Weibel lab protocols.<br> | ||
| + | Using this isolated DNA ran a pcr reaction as before and got one successful band at 700bp.<br> | ||
| + | We purified and stored this band at -20.<br> | ||
| + | After discussions with faculty new C-Media recipe was obtained.<br> | ||
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'''Team Fungus:''' <br> | '''Team Fungus:''' <br> | ||
Ran leftovers from yesterday's PCR in 1% agrose gel<br> | Ran leftovers from yesterday's PCR in 1% agrose gel<br> | ||
| - | Used QIAquick gel extraction microcentrifuge kit | + | -correct sized bands visible in UV light <br> |
| - | + | Used QIAquick gel extraction microcentrifuge kit and QIAquick PCR purification to get purified product<br> | |
| - | <br> | + | Used QIAprep miniprep kit to purify pET28a vectors from liquid LB + Kanamycin media that had incubated overnight<br> |
| - | Used QIAprep miniprep kit to purify pET28a vectors from liquid LB + Kanamycin media that had incubated overnight | + | Ran another touchdown PCR of both Carbon and Nitrogen limited cDNA using Herculase II polymerase <br> |
| - | <br> | + | Used QIAquick PCR purification kit to purify PCR product <br> |
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Latest revision as of 19:43, 18 October 2008
| Team Sorbitol:
Attempted to isolate genomic DNA from E. coli MG1655 using the Weibel lab protocols. Team Fungus: |
