Team:Warsaw/Calendar-Main/23 June 2008

From 2008.igem.org

(Difference between revisions)
Line 2: Line 2:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
<html>
<html>
 +
 +
 +
<h3>The analysis of beta-galactosidase sequence from pZC</h3>
 +
<h4>Piotr</h4>
 +
There is no mutation in beta-galactosidase gene. Why the colonys are white???
 +

Revision as of 18:26, 19 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


The analysis of beta-galactosidase sequence from pZC

Piotr

There is no mutation in beta-galactosidase gene. Why the colonys are white???

Preparation of constructs with OmpA protein fusions

Michał K.

Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha and pACYC177+OmpA_omega (liquid LB + kanamycin).

Preparation of alpha-A and omega-A fusions

Michał L., Ewa, Marcin

We have successfully amplified Omega-linker (Fig. 1), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48°C. Mg2+ concentration elevated to 4 mM.

Fig. 1.PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/23_June_2008"