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Team:Warsaw/Calendar-Main/23 June 2008
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<h3>Preparation of alpha-A and omega-A fusions</h3> | <h3>Preparation of alpha-A and omega-A fusions</h3> | ||
| - | <h4>Michał L. | + | <h4>Michał L.</h4> |
<p>We have successfully amplified Omega-linker (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/23_June_2008#fig1">Fig. 1</a>), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48°C. Mg<sup>2+</sup> concentration elevated to 4 mM.</p> | <p>We have successfully amplified Omega-linker (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/23_June_2008#fig1">Fig. 1</a>), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48°C. Mg<sup>2+</sup> concentration elevated to 4 mM.</p> | ||
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/36/PCR_Alinker_Omegalinker_WAW.jpg"width=200/></a> | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/36/PCR_Alinker_Omegalinker_WAW.jpg"width=200/></a> | ||
Revision as of 18:29, 19 October 2008
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The analysis of beta-galactosidase sequence from pZCPiotrThere is no mutation in beta-galactosidase gene. Why the colonys are white??? This is wery strange... We must make new reporter system.Preparation of constructs with OmpA protein fusionsMichał K.Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha and pACYC177+OmpA_omega (liquid LB + kanamycin). Preparation of alpha-A and omega-A fusionsMichał L.We have successfully amplified Omega-linker (Fig. 1), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48°C. Mg2+ concentration elevated to 4 mM. 
Fig. 1.PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.
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