Team:Warsaw/Calendar-Main/24 June 2008

From 2008.igem.org

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<h3>Blue/white and rifampicin test</h3>
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<h4>Michał L., Ewa, Marcin</h4>
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<p>Sequencing determined that all white colonies from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_June_2008">16 June</a> have intact lacZ gene. Reason of white phenotype unknown. We suppose that it is due to chromosomal mutation. We need another reporter system (not splitted to chromosomal and plasmid part --> GFP? ).</p>
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Revision as of 18:30, 19 October 2008

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Preparation of alpha-A and omega-A fusions

Michał L., Ewa, Marcin

Still no success. We need to run gradient PCR to find optimal reaction conditions.

Preparation of constructs with OmpA protein fusions

Michał K.

  1. PCR on isolated plasmids with OmpaL_N and OmpaP_link primers.
  2. Gel electrophoresis (Fig. 1, no proper clones found).

Fig. 1. PCR product - searching for fusions with OmpA protein. Lower lanes - OmpA_alpha, upper - OmpA_omega.

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