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Team:Warsaw/Calendar-Main/2 September 2008
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| - | <h3> | + | <h3>Pur<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A_alpha</a> in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain from overnight culture inoculated in fresh LB and cultured until OD=0,5.</li> |
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<li>Culture induced with different concentrations of IPTG in 22°C and 37°C.</li> | <li>Culture induced with different concentrations of IPTG in 22°C and 37°C.</li> | ||
<li>Samples were collected twice: after 3h and next day (samples were centrifuged and frozen).</li></ol></p> | <li>Samples were collected twice: after 3h and next day (samples were centrifuged and frozen).</li></ol></p> | ||
Revision as of 21:03, 19 October 2008
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PurpET15b+A_alpha in Rosetta strain from overnight culture inoculated in fresh LB and cultured until OD=0,5. |
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