Team:Warsaw/Calendar-Main/20 May 2008
From 2008.igem.org
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<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
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- | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of PCR product for transcriptional fusion (T7 RNA-polymerase) with HindIII and SalI (2x Tango buffer).</li> | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of PCR product for <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>transcriptional fusion</a> (T7 RNA-polymerase) with HindIII and SalI (2x Tango buffer).</li> |
- | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of PCR product for translational fusion (AID+T7 RNA-polymerase) with NcoI and XbaI (1x Tango buffer).</li> | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of PCR product for <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>translational fusion</a> (AID+T7 RNA-polymerase) with NcoI and XbaI (1x Tango buffer).</li> |
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of digested products from p.1 and p.2.</li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of digested products from p.1 and p.2.</li> |
Revision as of 21:28, 19 October 2008
Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7Michał K.
Rifampicin testMichał L., Ewa, MarcinPlating cultures from the previous day on rifampicin.
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