Team:Warsaw/Calendar-Main/21 May 2008

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<h4>Michał K.</h4>
<h4>Michał K.</h4>
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<li> Inoculation of separate transformant colonies for transcriptional fusion (liquid LB + tetracycline).</li>
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<li> Inoculation of separate transformant colonies for <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>transcriptional fusion</a> (liquid LB + tetracycline).</li>
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<li> Inoculation of separate transformant colonies for translational fusion (liquid LB + tetracycline). </li>
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<li> Inoculation of separate transformant colonies for <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>translational fusion</a> (liquid LB + tetracycline). </li>
</ol></p>
</ol></p>

Revision as of 21:29, 19 October 2008

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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

  1. Inoculation of separate transformant colonies for transcriptional fusion (liquid LB + tetracycline).
  2. Inoculation of separate transformant colonies for translational fusion (liquid LB + tetracycline).

Results of rifampicin test

Michał L., Ewa, Marcin

StrainInducer / repressorNumber of colonies on rifampicin
Top10none12
Top100.5 mM IPTG17
Top10 pMPM-AIDnone13
Top10 pMPM-AID0.1% Glucose18
Top10 pMPM-AID0.1% Arabinose54
Top10 pTRC-AIDnone14
Top10 pTRC-AID1% Glucose17
Top10 pTRC-AID0.5 mM IPTG53

Conclusion: AID seems to work!

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