Team:Warsaw/Calendar-Main/19 June 2008

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(Difference between revisions)
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<h4>Piotr</h4>
<h4>Piotr</h4>
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<li>The analysis of lacZ sequence from pZC:<br>
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<li>The analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br>
There is no mutation in beta-galactosidase gene. Why the colonys are white???</li>
There is no mutation in beta-galactosidase gene. Why the colonys are white???</li>
<li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a>.<br>
<li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a>.<br>

Revision as of 09:13, 20 October 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).

Blue/white test

Piotr

  • The analysis of lacZ sequence from pZC320:
    There is no mutation in beta-galactosidase gene. Why the colonys are white???
  • Colony PCR.
    Template: DNA isolated from white colonies
    Primers: pZCseqL and pZCseqR

    Colony PCR program
    TemperatureTimeNo. of cycles
    94°C4:00
    94°C0:3028 cycles
    48°C0:45
    72°C1:30
    72°C10:00
    4°Cinfinite


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