Team:Warsaw/Calendar-Main/25 August 2008

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<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4>
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Purification Omp_alpha of antibiotic resistance contamination
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<p> Inoculation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  careing pACTC177+OmpA_alpha.</p>
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<h3>Cloning of protein A DNA to <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+Omp-alpha</a> plasmid</h3><h4>Antoni</h4>
<h3>Cloning of protein A DNA to <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+Omp-alpha</a> plasmid</h3><h4>Antoni</h4>

Revision as of 18:23, 20 October 2008

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Tests for ampicillin resistance given by protein added to medium

Piotr

Purification Omp_alpha of antibiotic resistance contamination

Inoculation of E. coli TOP10 careing pACTC177+OmpA_alpha.

Cloning of protein A DNA to pET15b+Omp-alpha plasmid

Antoni

  1. Transformation of E. coli TOP10 strain with friday's ligation products.
  2. Transformants plating on LB + ampicillin.

Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega

Michał K.

Eight colonies from each plate (pACYC177+OmpA_alpha_A_omega and pACYC177+OmpA_alpha_Z_omega) was inoculated to liquid LB with kanamycin.

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