Brown: Team Resistance/9 July 2008

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==9 July 2008==
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*OPTICAL DENSITY TEST:
*OPTICAL DENSITY TEST:
*We made multiple solutions of pVJ4 and added varying amounts of Arabinose to determine how long complete lysis takes. We also added Chloramphenicol (1:1 ratio) in addition to Arabinose to see whether it would speed up lysis.
*We made multiple solutions of pVJ4 and added varying amounts of Arabinose to determine how long complete lysis takes. We also added Chloramphenicol (1:1 ratio) in addition to Arabinose to see whether it would speed up lysis.
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*Initial OD 50mL culture: .038
*Initial OD 50mL culture: .038
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<nowiki>   .2%(1)  .2%(2)  .4%(1)  .4%(2)  .2% + Chloramphenicol(1)  .2% + Chloramphenicol(2)  Control
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   .2%(1)  .2%(2)  .4%(1)  .4%(2)  .2% + Chloramphenicol(1)  .2% + Chloramphenicol(2)  Control
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3:45 PM    .084    .085      .079  .079      .062                        .053                  .073
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  1 .084    .085      .079  .079      .062                        .053                  .073
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4:45 PM    .065    .069      .040  .048      .044                        .047                  .076
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  2 .065    .069      .040  .048      .044                        .047                  .076
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5:55 PM    .018    .018      .023  .021      .034                        .050                  .075
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  3 .018    .018      .023  .021      .034                        .050                  .075
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6:50 PM    .014    .015      .013  .013      .050                        .044                  .071</nowiki>
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  4 .014    .015      .013  .013      .050                        .044                  .071
*The first few readings indicated an increase in the optical density.  This is to be expected because the bacteria are in the mid-log phase and still growing.  Growth must occur in order for the S, R, and Rz genes to be expressed and translated.  Cell lysis is complete in four hours.
*The first few readings indicated an increase in the optical density.  This is to be expected because the bacteria are in the mid-log phase and still growing.  Growth must occur in order for the S, R, and Rz genes to be expressed and translated.  Cell lysis is complete in four hours.
*The addition of Chloramphenicol did not alter the rate of lysis.
*The addition of Chloramphenicol did not alter the rate of lysis.

Latest revision as of 01:27, 21 October 2008


Toxipop lab notebook.png

9 July 2008

  • OPTICAL DENSITY TEST:
  • We made multiple solutions of pVJ4 and added varying amounts of Arabinose to determine how long complete lysis takes. We also added Chloramphenicol (1:1 ratio) in addition to Arabinose to see whether it would speed up lysis.
  • Initial OD 50mL culture: .038 
  .2%(1)   .2%(2)   .4%(1)  .4%(2)  .2% + Chloramphenicol(1)  .2% + Chloramphenicol(2)  Control
 1 .084     .085      .079   .079       .062                        .053                  .073
 2 .065     .069      .040   .048       .044                        .047                  .076
 3 .018     .018      .023   .021       .034                        .050                  .075
 4 .014     .015      .013   .013       .050                        .044                  .071


  • The first few readings indicated an increase in the optical density. This is to be expected because the bacteria are in the mid-log phase and still growing. Growth must occur in order for the S, R, and Rz genes to be expressed and translated. Cell lysis is complete in four hours.
  • The addition of Chloramphenicol did not alter the rate of lysis.
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