Team:Warsaw/Calendar-Main/17 September 2008

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	Attribution analyzer / Interactive list of topics

Competition tests

Piotr

Plasmid isolation from previous day's cultures.
Digest with SacI and BamHI.
Electrophoresis.

[photo of the gel is to be placed here]

Conclusion: cells with interracting protein survive competition!

MutD5 testing

Emilia

Inoculation of MutD5 into medium with tetracycline.

Optimisation of primers for preparation of parts

Michał K.

PCR on pACYC177+OmpA_omega_deltaA_alpha plasmid using AL_BNXNE and APSacSpe primers (elongation length 45s) to obtain deltaA fragment.
PCR on pACYC177+OmpA_omega_deltaA_alpha plasmid using LinLSXNE and AlfaPSpe primers (elongation length 60s) to obtain link_alpha fragment.
PCR on pACYC177 + OmpA_omega plasmid using LinLSXNE and OmegPSpe primers (elongation length 60s) to obtain link_omega fragment.
PCR on pACYC177+OmpA_omega_deltaA_alpha plasmid using OmLNXNEB and LinPBSNP primers (elongation length 90s) to obtain Omp_omega fragment.

Each reaction was carried out with 25 cycles and in temperature gradient from 55 to 75 °C.

@@ Line 20: / Line 20: @@
 <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL_BNXNE">AL_BNXNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#APSacSpe">APSacSpe</a>
-  primers (elongation length 45s). </li>
+  primers (elongation length 45s) to obtain deltaA fragment. </li>
 <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pACYC177+OmpA_omega_deltaA_alpha plasmid using
 <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinLSXNE">LinLSXNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlfaPSpe">AlfaPSpe</a>
-primers (elongation length 60s). </li>
+primers (elongation length 60s) to obtain link_alpha fragment. </li>
 <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega">pACYC177 + OmpA_omega</a> plasmid using
 <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinLSXNE">LinLSXNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegPSpe">OmegPSpe</a>
-primers (elongation length 60s). </li>
+primers (elongation length 60s) to obtain link_omega fragment. </li>
 <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pACYC177+OmpA_omega_deltaA_alpha plasmid using
 <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmLNXNEB">OmLNXNEB</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinPBSNP">LinPBSNP</a>
-primers (elongation length 90s). </li>
+primers (elongation length 90s) to obtain Omp_omega fragment. </li>
 <p>Each reaction was carried out with 25 cycles and in temperature gradient from 55 to 75 &deg;C.</p>