Team:Warsaw/Calendar-Main/25 September 2008

From 2008.igem.org

(Difference between revisions)
Line 19: Line 19:
<h3>Preparation of BioBricks</h3>
<h3>Preparation of BioBricks</h3>
<h4>Piotr</h4>
<h4>Piotr</h4>
-
<ol><li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a>
+
<ol><li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL_BNXNE">AL_BNXNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a>
  primers on colonies from plates with transformations OmpA_alpha (45 s of elongation step). </li>
  primers on colonies from plates with transformations OmpA_alpha (45 s of elongation step). </li>

Revision as of 20:08, 22 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Mutagenesis of protein A

Paweł

Treatment of mutageneses as on 23rd September.

Preparation of alpha_A construct

Antoni

  1. PCR on alpha_linker and linker_A with AlphaL+SacI and AP+NotI primers and 10% DMSO (30 cycles, elongation 60 s, annealing temperature 72°C).
  2. Gel electrophoresis of PCR products and gel-out of proper band (1019 bp).
  3. Measurment of concentration of both isolated products.

Preparation of BioBricks

Piotr

  1. Colony PCR with AL_BNXNE and OmpaP_link primers on colonies from plates with transformations OmpA_alpha (45 s of elongation step).
  2. Plating on LB + ampicillin.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/25_September_2008"