Team:Warsaw/Calendar-Main/25 September 2008

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(Difference between revisions)
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<h3>Preparation of BioBricks</h3>
<h3>Preparation of BioBricks</h3>
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<h4>michał K.</h4>
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<p> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL_BNXNE">AL_BNXNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#APSacSpe">APSacSpe</a>
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primers on colonies from plates with transformations RFP(??????????) + deltaA. No products visible.</p>
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<h4>Piotr</h4>
<h4>Piotr</h4>
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<ol><li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL_BNXNE">AL_BNXNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#APSacSpe">APSacSpe</a>
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<p>Inoculation of some colonies from plate to LB with ampicillin</p>
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primers on colonies from plates with transformations RFP(??????????) + deltaA. No products visible.</li>
 
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<li>Inoculation of some colonies from plate to LB with ampicillin</li>
 
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</ol>
 

Revision as of 20:13, 22 October 2008

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Mutagenesis of protein A

Paweł

Treatment of mutageneses as on 23rd September.

Preparation of alpha_A construct

Antoni

  1. PCR on alpha_linker and linker_A with AlphaL+SacI and AP+NotI primers and 10% DMSO (30 cycles, elongation 60 s, annealing temperature 72°C).
  2. Gel electrophoresis of PCR products and gel-out of proper band (1019 bp).
  3. Measurment of concentration of both isolated products.

Preparation of BioBricks

michał K.

Colony PCR with AL_BNXNE and APSacSpe primers on colonies from plates with transformations RFP(??????????) + deltaA. No products visible.

Piotr

Inoculation of some colonies from plate to LB with ampicillin

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