Origami-DNA

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Revision as of 20:02, 23 October 2008

_DNA-Origami

Introduction

Paul Rothemund has discovered that it is possible to shape M13-Phage single-strand-DNA simply adding oligonucleotides that will work as „brackets“ when complementing the long single-strand.
In this way, one can generate for example DNA-squares of a certain size with „nods“ at certain distances. One member of our team, Daniel Hautzinger, has recently finished his diploma-thesis on Origami-DNA and the possibilities of generating patterns on these square surfaces by modifying the oligo-nucleotides that build up the nod-points.
As the antigens NIP and fluoresceine can as well be fused to these oligos, we had found the seemingly perfect tool to present strictly defined two-dimensional antigen-patterns to cells carrying our synthetic receptor system. File:Freiburg2008 NIP arrangement schematical.jpg

Literature

Paul W. K. Rothemund: Nature 440, 297-302 (16 March 2006)

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 As the antigens NIP and fluoresceine can as well be fused to these oligos, we had found the seemingly perfect tool to present strictly defined two-dimensional antigen-patterns to cells carrying our synthetic receptor system.
 [[Image:Freiburg2008_Fab_on_Origami_animated.gif|800 px]]
+[[Image:Freiburg2008_NIP_arrangement_schematical.jpg]]
 ==Literature==
 *Paul W. K. Rothemund: Nature 440, 297-302 (16 March 2006)
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