Team:Warsaw/Calendar-Main/29 September 2008

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<li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (Z - 200 bp and both RFP (??????)'s - 2200 bp).</li>
<li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (Z - 200 bp and both RFP (??????)'s - 2200 bp).</li>
<li> Gel electrophoresis to estimate concentration of purified DNA from previous day. </li>
<li> Gel electrophoresis to estimate concentration of purified DNA from previous day. </li>
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<li> Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments: RFP(??????) + Z and RFP(????) + OmpA_link (from 18 September). </li></ol>
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Revision as of 12:33, 24 October 2008

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Preparation of BioBricks

Michał K.

  1. Digest of Z-GeneArt and RFP(????)+deltaA plasmids with NdeI and BcuI (BamHI buffer).
  2. Digest of RFP(????)+deltaA plasmid with NdeI and SacI (BamHI buffer).
  3. Gel electrophoresis of digested plasmids and gel-out of proper bands (Z - 200 bp and both RFP (??????)'s - 2200 bp).
  4. Gel electrophoresis to estimate concentration of purified DNA from previous day.
  5. Overnight ligation of isolated DNA fragments: RFP(??????) + Z and RFP(????) + OmpA_link (from 18 September).




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