Team:Warsaw/Calendar-Main/29 September 2008
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<li> Gel electrophoresis to estimate concentration of purified DNA from previous day. </li> | <li> Gel electrophoresis to estimate concentration of purified DNA from previous day. </li> | ||
<li> Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments: RFP(??????) + Z and RFP(????) + OmpA_link (from 18 September). </li> | <li> Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments: RFP(??????) + Z and RFP(????) + OmpA_link (from 18 September). </li> | ||
- | <li></ol> | + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pACYC177+OmpA_omega_deltaA_alpha plasmid using |
+ | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmLNXNEB">OmLNXNEB</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinPBSNP">LinPBSNP</a> | ||
+ | primers (annealing temperature 58 °C; elongation length 90s) to obtain Omp_omega fragment. </li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pACYC177+OmpA_omega_deltaA_alpha plasmid using | ||
+ | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#PlacL_XNE">PlacL_XNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinP_BS">LinP_BS</a> | ||
+ | primers (annealing temperature 58 °C; elongation length 90s) to obtain Omp_omega fragment. </li> | ||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pACYC177+OmpA_omega_deltaA_alpha plasmid using | ||
+ | |||
+ | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL_BNXNE">AL_BNXNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#APSacSpe">APSacSpe</a> | ||
+ | |||
+ | primers (annealing temperature 58 °C; elongation length 45s) to obtain deltaA fragment. </li> | ||
+ | |||
+ | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega">pACYC177 + OmpA_omega</a> plasmid using | ||
+ | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinLSXNE">LinLSXNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegPSpe">OmegPSpe</a> | ||
+ | primers (annealing temperature 55 °C; elongation length 60s) to obtain link_omega fragment. </li> | ||
+ | </ol> | ||
<h4>Piotr</h4> | <h4>Piotr</h4> |
Revision as of 13:16, 24 October 2008
Preparation of BioBricksMichał K.
PiotrInoculation of plasmids received from iGEM HQs ( ).
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