Team:Warsaw/Calendar-Main/9 June 2008

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Latest revision as of 15:15, 24 October 2008


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Digest of confirmed plasmid pMPMT5-AID+AID-T7 with HindIII and SalI (2x Tango buffer).
DNA gel electrophoresis.
Isolation of DNA from proper band (3300 bp).
Sequencing of isolated band with AIDseqP and T7Rseq.

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+<html><h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID%2BAID-T7>pMPMT5-AID+AIDT7</a> construct</h3>
+<h4>Michał K.</h4>
+<ol>
+<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of confirmed plasmid <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID%2BAID-T7>pMPMT5-AID+AID-T7</a> with HindIII and SalI (2x Tango buffer).</li>
+<li>DNA gel electrophoresis.</li>
+<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Isolation of DNA</a> from proper band (3300 bp).</li>
+<li> Sequencing of isolated band with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDseqP">AIDseqP</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7Rseq">T7Rseq</a>. </li></ol>
+</html>
-<p> Inoculation of 5 colonies of transformants (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion) into liquid LB broth.<br>
-Inoculation of 5 colonies of transformants (pMPM-T5 + T7 RNA-polymerase) into liquid LB broth.</p>
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