Team:Warsaw/Calendar-Main/31 July 2008
From 2008.igem.org
(Difference between revisions)
Line 28: | Line 28: | ||
<img src="https://static.igem.org/mediawiki/2008/e/e7/July_31_st.jpg" width=350 /><var><b>Z-omega induction with various IPTG concentrations<br> | <img src="https://static.igem.org/mediawiki/2008/e/e7/July_31_st.jpg" width=350 /><var><b>Z-omega induction with various IPTG concentrations<br> | ||
- | The arrow shows place of our overexpressed protein</b>: | + | The arrow shows place of our overexpressed protein</b><br>: |
1. 22°C 0,5 mM IPTG<br> | 1. 22°C 0,5 mM IPTG<br> | ||
2. 22°C 0,1 mM IPTG<br> | 2. 22°C 0,1 mM IPTG<br> |
Revision as of 15:19, 24 October 2008
Purification of proteins: Z-alpha and Z-omegaPiotr, Emilia
Cloning of omega_A DNA fragment to pACYC177+OmpA_alphaMichał K.
Cloning of truncated fragment of protein AMichał K.The arrow shows place of our overexpressed protein : 1. 22°C 0,5 mM IPTG 2. 22°C 0,1 mM IPTG 3. 22°C 1 mM IPTG 4. Marker 5. negative control 6. 37°C 0,1 mM IPTG 7. 37°C 0,5 mM IPTG 8. 37°C 1 mM IPTG
|