Team:Warsaw/Calendar-Main/2 July 2008

From 2008.igem.org

(Difference between revisions)
Line 3: Line 3:
<html>
<html>
-
<h3>Change of the reporter from pZC with B-galactosidaze to GFP or RFP</h3>
+
<h3>Change of the reporter from <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with B-galactosidase to GFP or RFP</h3>
<h4>Piotr, Weronika</h4>
<h4>Piotr, Weronika</h4>
<ol>
<ol>
Line 9: Line 9:
<li>
<li>
-
Gel electrophoresis and gel out of proper bands
+
Gel electrophoresis and gel-out of proper bands
</li>
</li>
<li>
<li>
-
Ligation of pZC with nazwa (Gfp genetrator), nazwa (RFP generator)  
+
Ligation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with standard parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>(Gfp genetrator) and <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450>BBa_J04450</a> (RFP generator)  
</li>
</li>

Revision as of 16:37, 24 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Change of the reporter from pZC320 with B-galactosidase to GFP or RFP

Piotr, Weronika

  1. Gel electrophoresis and gel-out of proper bands
  2. Ligation of pZC320 with standard parts: BBa_E0840(Gfp genetrator) and BBa_J04450 (RFP generator)
  3. Chemotransformation of E.coli TOP10 with ligation products
  4. Plating transformants on LB+Amp30+X-gal+IPTG

    Preparation of constructs with OmpA protein fusions

    Paweł

    1. Clean-up of overnight digest.
    2. Ligation of OmpA_alfa and pET15b.
    3. Ligation of OmpA_omega and pET15b.
    4. Transformation of E. coli TOP10 strain with ligation products.
    5. Transformants plating on LB + ampicillin.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/2_July_2008"