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Team:Tokyo Tech/Construction
From 2008.igem.org
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Revision as of 17:17, 24 October 2008
| Home | Construction | Acrylic container | Development of promoter | Genetic toggle switch | Parts Submitted to the Registry | Result | The Team | Notebook |
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Construction
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For checking pressure-response ability of Plac, we constructed three plasmids.Those are showed at figure 1.Plac stands for a promoter repressed by lacI protein. Ptet stands for promoter repressed by tetR protein.∆p stands for promoter less.JM109 is constitutive expression lacI protein so that Plac is always repressed by lacI protein.And JM109 isn’t expression tetR. Therefore, we used Ptet as a positive control. |
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Fig1 |
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Each parts is constructed on low-copy plasmid “pSB6” ,and inserted into E.coli strain JM109. |
