Team:Warsaw/Calendar-Main/3 October 2008

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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmid with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol>
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmid with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol>
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<h3></h3>
<h4>Piotr</h4>
<h4>Piotr</h4>
<ol><li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of TOP10 with ligations (3kb??????) + _alpha, (3kb????) + _omega and pACYC177 + OmpA_omega_ (both digested with BamHI and PstI - from 30 September).</li>
<ol><li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of TOP10 with ligations (3kb??????) + _alpha, (3kb????) + _omega and pACYC177 + OmpA_omega_ (both digested with BamHI and PstI - from 30 September).</li>

Revision as of 18:06, 24 October 2008

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Preparation of BioBricks

Michał K.

  1. Isolation of plasmid from culture inoculated on previous day (RFP(?????)+Z).
  2. Control digest of isolated plasmid with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

Piotr

  1. Transformation of TOP10 with ligations (3kb??????) + _alpha, (3kb????) + _omega and pACYC177 + OmpA_omega_ (both digested with BamHI and PstI - from 30 September).
  2. Planting on LB with kanamycin.

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