Team:Warsaw/Calendar-Main/11 September 2008

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<p><ol><li>Inoculation of OmpA_variant from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicylin with minimum concentration of prey (yesterday results).</li>
<p><ol><li>Inoculation of OmpA_variant from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicylin with minimum concentration of prey (yesterday results).</li>
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<li>Mesurment of growth after 6 hours - 2 fold digger growth with interacting proteins (Omp_A_omega 2x more than Omp_omega with protein Z_alpha etc..)!!!
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<li>Mesurment of growth after 6 hours - 2 fold digger growth with interacting proteins (Omp_A_omega twice as more as Omp_omega with protein Z_alpha etc..)!!!

Revision as of 18:13, 24 October 2008

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Tests for ampicillin resistance given by protein added to medium

Piotr

  1. Inoculation of OmpA_variant from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicylin with minimum concentration of prey (yesterday results).
  2. Mesurment of growth after 6 hours - 2 fold digger growth with interacting proteins (Omp_A_omega twice as more as Omp_omega with protein Z_alpha etc..)!!!


    We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!

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